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•             Question 1
                When performing the urease test, which of the following color changes is expected if the organism is positive for urease production?                                       
               
Answers:              
The agar changes from yellow to bright pink
                The agar changes from red to yellow
                The agar changes from blue to green
                The agar changes from colorless to black
                                               
•             Question 2
 
               
                The oxidase test determines the presence of cytochrome oxidase enzyme. This enzymes functions as part of _____________                                            
               
Answers:              
The electron transport chain
                Glycolysis
                The citric acid cycle
                Fermentation
                                               
•             Question 3
 
               
                Genotypic identification methods examine the DNA sequence of an organism                                  
               
Answers:               True
                False
                                               
•             Question 4
 
               
                Which of the following would be most valuable to confirm the identification of microorganisms isolated during a food poisoning outbreak?                                           
               
Answers:             Growth on agar plates to identify colony morphology
                Grain stain to view cellular morphology
                 
Identification of microorganisms using microscopic, cultural, and physiological characteristics
                                               
•             Question 5
 
               
                Which of the following are examples of physiological (biochemical) characteristics used in the bacterial identification process?                                 
               
Answers:             Gram stain
                Cell shape
                 
Presence of a specific enzyme
                Growth in a nutrient broth
                                               
•             Question 6
 
               
                Which methods used in bacterial identification usually require culturing of the unknown bacteria?                                          
Answers:             Immunologic
                Genotypic
                 
Phenotypic
                Morphologic
                                               
•             Question 7
 
               
                If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test will be red, indicating a positive result.                                         
               
Answers:               True
                False
                                               
•             Question 8
 
               
                Which reagent is added to SIM medium to detect indole production?                                    
Answers:             Methyl red reagent
                Phenol red
                Zinc powder
                 
Kovac’s reagent
                                               
•             Question 9
 
               
                In the Kligler’s Iron Agar (KIA) test, if an organism can only ferment glucose, the butt of the agar slant turns ______, and the slanted surface of the agar will be _______ after 24 hours of incubation.                                          
Answers:             black; yellow
                Red; yellow
                 
Yellow; red
                Yellow; black
                                               
•             Question 10
 
               
                All of the following biochemical tests involve the growth of an organism in a specific liquid broth medium EXCEPT                                               
Answers:             Nitrate reduction test
                 
SIM test
                Phenol red carbohydrate fermentation test
                Methyl red test
                                               
•             Question 11
 
               
                Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar.                                               
               
Answers:             They are glucose fermenters
                 
They are lactose fermenters
                They produce urease
                They reduce nitrate to nitrite
                                               
•             Question 12
 
               
                The internationally recognized reference for bacterial classification and identification is called Bergey’s Manual                                 
               
Answers:               True
                False
                                               
•             Question 13
 
               
                An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____ characteristics that can be helpful in the identification of the microorganism.                                       
Answers:             Morphological
                Genotypic
                 
Physiological
                Immunological
                                               
•             Question 14
 
               
                Which of the following types of media allows for the observations of multiple biochemical properties in one?                                    
Answers:             Urea agar
                MacConkey agar
                 
SIM media
                Citrate media
                                               
•             Question 15
                Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT          Answers:              
Nitrate reduction
                Acid production
                Gas production
                Fermentation

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